Rop1Ps promote actin cytoskeleton dynamics and control the tip growth of lily pollen tube |
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Authors: | Heping Zhao Haiyun Ren |
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Institution: | (1) Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, Beijing Normal University, 100875 Beijing, PRChina |
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Abstract: | Rop, the small GTPase of the Rho family in plants, is believed to exert molecular control over dynamic changes in the actin
cytoskeleton that affect pollen tube elongation characteristics. In the present study, microinjection of Rop1Ps was used to
investigate its effects on tip growth and evidence of interaction with the actin cytoskeleton in lily pollen tubes. Microinjected
wild type WT-Rop1Ps accelerated pollen tube elongation and induced actin bundles to form in the very tip region. In contrast,
microinjected dominant negative DN-rop1Ps had no apparent effect on pollen tube growth or microfilament organization, whereas
microinjection of constitutively active CA-rop1Ps induced depolarized growth and abnormal pollen tubes in which long actin
bundles in the shank of the tube were distorted. Injection of phalloidin, a potent F-actin stabilizer that inhibits dynamic
changes in the actin cytoskeleton, prevented abnormal growth of the tubes and suppressed formation of distorted actin bundles.
These results indicate that Rop1Ps exert control over important aspects of tip morphology involving dynamics of the actin
cytoskeleton that affect pollen tube elongation.
Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users. |
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Keywords: | Small GTPase Rop Microinjection Pollen tube growth Actin cytoskeleton |
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