The efficacy of new colchicine derivatives and viability of the T-Lymphoblastoid cells in three-dimensional culture using F MRI and HPLC-UV ex vivo

The paper describes ex vivo applications of colchicine derivatives for the treatment of human T-Lymphoblastoid (CEM) cells. Moreover, the role of the substitutions of ring A at C-1 and C-7 side chain of colchicine analogues was probed by the synthesis and examination of their effects on the three-dimensional (3-D) CEM cells’ growth. The CEM cells were cultured in the hollow fiber bioreactor (HFB) device. We used ¹H and ¹⁹F magnetic resonance imaging (MRI) to monitor changes in 3-D CEM cell culture. ¹⁹F MRI was used for visualization of the cellular uptake of new fluorine derivatives. Before and after treatment CEM cells profile was investigated with high performance liquid chromatography (HPLC-UV).