Diacetyl production by co-immobilized citrate-positiveLactococcus
lactis subsp.lactis 3022 and homogenized bovin liver in alginate fibers with double gel layers |
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Authors: | H Ochi M Takahashi T Kaneko H Suzuki H Tanaka |
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Institution: | (1) Institute of Applied Biochemistry, University of Tsukuba, 305 Tsukuba, Ibaraki, Japan;(2) Central Research Institute, Meiji Milk Products Co., Ltd., Higashimurayama, 189 Tokyo, Japan |
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Abstract: | Summary Diacetyl production by (Citr+)Lactococcus
lactis subsp.lactis 3022 cells immobilized in Ca-alginate fine fibers with single layer in the presence of catalase was three times higher than that in the absence of catalase. A co-immobilized culture system of the lactic acid bacterial cells (outer) and the homogenized bovine liver (inner layer) in Ca-alginate fibers with double gel layers was developed. The culture system gave high diacetyl productivity (30 mg/l) for ten repeated batch cultures. |
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