Ultrastructure and genotypic characterization of the filamentous fungus Cochliobolus lunatus in comparison to the anamorphic strain Curvularia lunata |
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Authors: | Damjana Rozman Kristijan Jezernik Radovan Komel |
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Affiliation: | Institute of Biochemistry, Medical Faculty, Vrazov trg 2, 61000 Ljubljana, Slovenia; Institute of Cell Biology, Medical Faculty, Vrazov trg 2, 61000 Ljubljana, Slovenia |
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Abstract: | Abstract Although Curvularia lunata is classified as a conidial anamorph of Cochliobolus lunatus , the electron microscopic studies revealed the ultrastructure of both strains to be significantly different. C. lunatus m118 grows in the hyphal form characterized by a thick cell wall and numerous lipid bodies. C. lunata forms thinner hyphae of various sizes and large oval spores. Electrophoretic karyotypes of C. lunatus m118 (this paper) and C. lunata AT46 (Osiewacz, H. and Ridder, R. (1991) Curr. Genet. 20, 151–155) as well as RAPD-PCR analysis with the primer (GTG)5 indicate close genetic relationship of both microorganisms. |
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Keywords: | Cochliobolus lunatus Curvularia lunata Filamentous fungi RAPD-PCR Electrophoretic karyotype |
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