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Cell separation from high cell density broths of Alcaligenes eutrophus by using a coagulant |
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| Authors: | Hee Wook Ryu Kyung Suk Cho Young Keun Chang Ho Nam Chang |
| Affiliation: | (1) Department of Chemical Engineering, Soong Sil University, 1-1 Sangdo-5 dong, 156-743 Dongjak-gu, Seoul, Korea;(2) Department of Environmental Engineering, Ewha Womans University, 11-1 Daehyun-dong, 120-750 Seodaemun-gu, Seoul, Korea;(3) Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, 305-701 Yusong-gu, Taejon, Korea |
| Abstract: | Summary Alcaligenes eutrophus was successfully recovered from high cell density broths by pre-treatment with polyaluminium hydroxide chloride silicate as a coagulant at 36–90 mg Al/l. The optimum pH range for cell coagulation was 10–12. Subsequent centrifugation (45×g) and filtration (pore size 0.5 mm) gave a cell recovery of higher than 90%. The energy demand for cell recovery with the coagulant was only 3–11% of that without it. |
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