不同日龄小鼠的超排、体外授精及二细胞胚胎移植的比较研究(简报)

小鼠是现代生物医学中应用最为广泛的实验动物,特别是随着基因工程动物的大量开发,对小鼠卵母细胞和小鼠胚胎的需求量将日趋增多。但是如果单纯依靠动物的自然排卵,一方面所得到的卵母细胞数或胚胎数较少,一方面又消耗大量的时间和浪费较多的动物。因此,研究者期望使用较少的动物但仍能得到较多的、正常的卵母细胞或胚胎来满足生命科学研究的需要。Onadera,M等的研究表明,小鼠卵母细胞的发育与年龄有关,同一品系小鼠不同日龄间的自然排卵数差异不显著,出现异常卵的比例有显著性差异。但对不同日龄、不同品系小鼠的超排的效果尚

THE COMPARATIVE STUDY ON OVA SUPEROVULATED, IN VITRO FERTILIZATION AND RATE OF PREGNANCY AT DIFFERENT AGES

The development of oocytes, superovulated at 28, 56 and 112 days in three mice strains (ICR, B6C3F1, and C57BL/6J) with PMSG and HCG, were examined using the techniques of in vitro fertilization, culture, and transfer of these two-cell embryos to pseudopregnant recipients. The highest numbers of oocytes were obtained from superovulated 28-day-old mice in three strains. Ap-proximately 90% of oocytes developed to the two-cell stage after in vitro fertilization, and about 50% became pregnant through the recipients. These results suggested that donor age at 28 days had prominent discrepancy with 56 and 112-day-old mice (P<0.01) in oocytes superovu-lation, and no influence on the rate of insemination and pregnancy.