| 芦笋雌性特异性片段的克隆和分析(简报) |
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| 引用本文： | 卢龙斗,李瑞丽,高武军,邓传良,王连军.芦笋雌性特异性片段的克隆和分析(简报)[J].分子细胞生物学报,2006,39(3):281-284. |
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| 作者姓名： | 卢龙斗 李瑞丽 高武军 邓传良 王连军 |
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| 作者单位： | 河南师范大学生命科学学院 新乡453007 |
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| 基金项目： | 河南省自然科学基金项目(编号:0324160310)，河南省植物学省级重点学科资助 |
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| 摘 要： | 芦笋(Asparagus officinalis L.)又名石刁柏、龙须菜,系雌雄异株宿根性植物,是重要的经济作物之一。芦笋的性染色体为一对同形的L5染色体,雌性的性染色体为XX,雄性的性染色体为XY。性别决定的多态性是由位于一对L5性染色体上的一个显性基因M决定的1-3],雌株基因型为隐性纯合子mm,雄株为显性纯合子MM(又称超雄株)或杂合子Mm。在生产上,由于雄株比雌株高产25%以上4],并具有极强的抗病性和生命力,故雄株特别是超雄株则倍受生产者的青睐,但芦笋雌雄鉴定只有等到种植的第二年待植株开花时才能进行,这就严重影响了芦笋的有目的种植和经济效…
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| 关 键 词： | 芦笋 性别 RAPD SCAR |
| 收稿时间： | 2005-11-07 |
| 修稿时间： | 2006-03-05 |
CLONING AND ANALYZING OF THE FEMALE-SPECIFIC MARKER IN THE DIOECIOUS SPECIES ASPARAGUS OFFICINALIS L. |
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| LU Long Dou,LI Rui Li,GAO Wu Jun,DENG Chuan Liang,WANG Lian Jun.CLONING AND ANALYZING OF THE FEMALE-SPECIFIC MARKER IN THE DIOECIOUS SPECIES ASPARAGUS OFFICINALIS L.[J].Journal of Molecular Cell Biology,2006,39(3):281-284. |
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| Authors: | LU Long Dou LI Rui Li GAO Wu Jun DENG Chuan Liang WANG Lian Jun |
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| Institution: | College of life science ,Henan Normal University, Xinxiang 453007 |
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| Abstract: | Sex-linked molecular markers are being obtained,which would be essential to be used in the screening of different sex of dioecious plants at the seedling stage. Furthermore,it is important in cloning the gene related to the sex. In this study the random amplified polymorphic DNA (RAPD) technique was employed with the objective to find markers linked to sex determination in Asparagus. A total of 100 primers were tested with the same PCR cycling procedure. A female-associated fragment with a length of about 867bp was generated with S12 primer. The fragment was cloned and sequenced,showing it is abundant in AT and contains 2 shorter open reading frames. In order to convert the RAPD marker into SCAR (sequence characterized amplified regions) marker,24bp specific primers were constructed and used for PCR amplifying. The female-linked dominant SCAR marker was obtained,which would be efficient to identify the different sex of Asparagus officinalis L. |
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| Keywords: | Asparagus officinalis L Sexuality RAPD SCAR |
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