Effect of irradiation intensity on cell growth and kalata B1 accumulation in <Emphasis Type="Italic">Oldenlandia affinis</Emphasis> cultures |
| |
Authors: | Heike Dörnenburg Peter Seydel |
| |
Institution: | (1) Institute of Bioprocess Engineering, University of Erlangen-Nuremberg, Paul-Gordan-Str. 3, 91052 Erlangen, Germany |
| |
Abstract: | Light irradiation had remarkable effects on callus growth of Oldenlandia affinis with an optimum intensity of 35 μmol m−2 s−1. Biosynthesis of kalata B1, the main cyclic peptide in O. affinis, was induced and triggered with rising irradiation intensities. The highest concentration of kalata B1, 0.49 mg g−1 DW characterised by the maximum productivity of 3.88 μg per litre and day was analysed at 120 μmol m−2 s−1, although callus growth was repressed. The light saturation point was established to be 35 μmol m−2 s−1, where kalata B1 productivity was in a similar order (3.41 μg per day) due to the higher growth index. O. affinis suspension cultures were shown to accumulate comparable specific kalata B1 concentrations in a delayed growth associated
production pattern. These were dependent on irradiation intensity (0.16 mg g−1 at 2 μmol m−2 s−1; 0.28 mg g−1 at 35 μmol m−2 s−1). The batch cultivation process resulted in a maximum productivity of 27.30 μg per litre and day with culture doubling times
of 1.16 d−1. Submers operation represented a 8-fold product enhancement compared to callus cultivation. |
| |
Keywords: | Oldenlandia affinis Callus cultures Suspension cultures Cyclotides kalata B1 Light stress |
本文献已被 SpringerLink 等数据库收录! |