Active glycolysis and glycogenolysis in early stages of primary cultured hepatocytes. Role of AMP and fructose 2.6-bisphosphate |
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Authors: | M Pilar López M Jose Gómez-Lechón Jose V Castell |
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Institution: | (1) Centro de Investigación, Hospital La Fe, INSALUD, Avda. Campanar 21, 46009 Valencia, Spain |
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Abstract: | Summary This study examines the factors involved in the rapid glycolysis and glycogenolysis that occur during the first stages of
hepatocyte culture: a) Shortly after seeding glycolysis, estimated as lactate released to culture medium, increased 10 times
in comparison to that reported in vivo. By 8 to 9 h of culture, hepatocytes were nearly glycogen-depleted even in the presence
of insulin. b) 6-Phosphofructo-2-kinase remained 100% active during this period. The proportion of the initial active phosphorylase
(87%) decreased to 57% by 7 h of culture. c) Fructose 2,6-bisphosphate content was initially similar to that found in liver
of fed animals, decreased after seeding and increased thereafter up to four times the initial concentration. In spite of changes
in the concentration of this activator, the glycolytic rate remained high and constant. d) ADP and AMP increased sharply after
cell plating, reaching values 1.7 and 3.5 times higher. The rise in AMP levels may be involved in the activation of glycolysis
and glycogenolysis, because this metabolite is known to act as an allosteric activator of phosphofrucktokinase and glycogen
phosphorylase. This metabolic situation resembles that of cells under hypoxia.
Part of this work was presented at the 38th Annual Meeting of the Tissue Culture Association, Washington, DC, May 1987. |
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Keywords: | hepatocytes serum-free culture glycogenolysis glycolysis fructose 2 6-bisphosphate AMP |
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