| 超滤法提纯万古霉素的应用 |
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| 引用本文： | 王健,张雪霞,王海燕,李晓露,张金娟,张丽.超滤法提纯万古霉素的应用[J].生物加工过程,2010,8(4):17-21. |
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| 作者姓名： | 王健 张雪霞 王海燕 李晓露 张金娟 张丽 |
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| 作者单位： | 华北制药集团,新药研究开发有限责任公司,微生物药物国家工程研究中心,石家庄,050015 |
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| 基金项目： | 国家科技支撑计划资助项目 |
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| 摘 要： | 采用Ultra-flo超滤系统提纯未经任何预处理的万古霉素发酵液。结果表明:万古霉素收率由传统工艺(板框加压过滤)的87%提高到94%,滤液的透光度比传统工艺提高了25%;系统平均膜通量可达120L/(m2.h);被污染的膜经清洗后与新膜没有明显差异;合理加水量为投料量的2.5倍。Ultra-flo超滤系统完全能代替传统工艺。
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| 关 键 词： | 发酵液 提纯 超滤 万古霉素 |
Purification of vancomycin by ultrafiltration |
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| WANG Jian,ZHANG Xue-xia,WANG Hai-yan,LI Xiao-lu,ZHANG Jin-juan,ZHANG Li.Purification of vancomycin by ultrafiltration[J].Chinese Journal of Bioprocess Engineering,2010,8(4):17-21. |
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| Authors: | WANG Jian ZHANG Xue-xia WANG Hai-yan LI Xiao-lu ZHANG Jin-juan ZHANG Li |
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| Institution: | (National Microbial Medicine Engineering ＆ Research Center,New Drug Research ＆ DevelopmentCompany of NCPC,Shijiazhuang 050015,China) |
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| Abstract: | Purification vancomycin from fermentation broth by ultrafiltration was investigated.The Ultra-Flo membrane system was used without pretreatment of the fermentation broth.The yield of vancomycin was 87% to 94% and the penetrability of filter liquid enhanced 25% compared with the traditional process.Membrane flux was 120 L/（m2·h）.There was no significant difference between new and contaminated membranes after clearing.The rational quantity of water used in filtering was 2.5 times than that of the original feed. |
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| Keywords: | fermentation broth purification ultrafiltration vanconmycin |
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