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Marine Bacteroidetes enzymatically digest xylans from terrestrial plants |
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| Authors: | Theresa Dutschei Irena Beidler Daniel Bartosik Julia-Maria Seeßelberg Michelle Teune Marcus Bäumgen Soraia Querido Ferreira Julia Heldmann Felix Nagel Joris Krull Leona Berndt Karen Methling Martin Hein Dörte Becher Peter Langer Mihaela Delcea Michael Lalk Michael Lammers Matthias Höhne Jan-Hendrik Hehemann Thomas Schweder Uwe T Bornscheuer |
| Institution: | 1. Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University Greifswald, Greifswald, Germany;2. Department of Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, Greifswald, Germany
Contribution: Data curation (equal), Formal analysis (equal), ?Investigation (equal), Methodology (equal), Visualization (equal), Writing - review & editing (equal);3. Department of Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, Greifswald, Germany Institute of Marine Biotechnology e.V., Greifswald, Germany Contribution: Data curation (equal), Formal analysis (equal), ?Investigation (equal), Software (equal), Visualization (equal);4. Department of Protein Biochemistry, Institute of Biochemistry, University of Greifswald, Greifswald, Germany;5. Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University Greifswald, Greifswald, Germany Contribution: Formal analysis (equal), ?Investigation (equal);6. Department of Biophysical Chemistry, Institute of Biochemistry, University of Greifswald, Greifswald, Germany Contribution: Formal analysis (equal), ?Investigation (equal);7. Institute of Marine Biotechnology e.V., Greifswald, Germany Center for Marine Environmental Sciences, University of Bremen, Bremen, Germany Contribution: Formal analysis (equal), ?Investigation (equal), Methodology (equal);8. Department of Synthetic and Structural Biochemistry, Institute of Biochemistry, University of Greifswald, Greifswald, Germany Contribution: Formal analysis (equal), ?Investigation (equal);9. Department of Cellular Biochemistry and Metabolomics, Institute of Biochemistry, University of Greifswald, Greifswald, Germany Contribution: Formal analysis (equal), ?Investigation (equal), Methodology (equal);10. Department of Organic Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany Contribution: Formal analysis (equal), ?Investigation (equal);11. Department of Microbial Proteomics, Institute of Microbiology, University of Greifswald, Greifswald, Germany Contribution: Funding acquisition (equal), Methodology (equal), Resources (equal);12. Department of Organic Chemistry, Institute of Chemistry, University of Rostock, Rostock, Germany Contribution: Formal analysis (equal), ?Investigation (equal), Methodology (equal);13. Department of Biophysical Chemistry, Institute of Biochemistry, University of Greifswald, Greifswald, Germany Contribution: Methodology (equal), Resources (equal);14. Department of Cellular Biochemistry and Metabolomics, Institute of Biochemistry, University of Greifswald, Greifswald, Germany Contribution: Data curation (equal), ?Investigation (equal), Methodology (equal);15. Department of Synthetic and Structural Biochemistry, Institute of Biochemistry, University of Greifswald, Greifswald, Germany Contribution: ?Investigation (equal), Methodology (equal);16. Institute of Marine Biotechnology e.V., Greifswald, Germany Center for Marine Environmental Sciences, University of Bremen, Bremen, Germany Contribution: Funding acquisition (equal), ?Investigation (equal), Methodology (equal), Resources (equal), Writing - review & editing (equal);17. Department of Pharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, Greifswald, Germany |
| Abstract: | Marine Bacteroidetes that degrade polysaccharides contribute to carbon cycling in the ocean. Organic matter, including glycans from terrestrial plants, might enter the oceans through rivers. Whether marine bacteria degrade structurally related glycans from diverse sources including terrestrial plants and marine algae was previously unknown. We show that the marine bacterium Flavimarina sp. Hel_I_48 encodes two polysaccharide utilization loci (PULs) which degrade xylans from terrestrial plants and marine algae. Biochemical experiments revealed activity and specificity of the encoded xylanases and associated enzymes of these PULs. Proteomics indicated that these genomic regions respond to glucuronoxylans and arabinoxylans. Substrate specificities of key enzymes suggest dedicated metabolic pathways for xylan utilization. Some of the xylanases were active on different xylans with the conserved β-1,4-linked xylose main chain. Enzyme activity was consistent with growth curves showing Flavimarina sp. Hel_I_48 uses structurally different xylans. The observed abundance of related xylan-degrading enzyme repertoires in genomes of other marine Bacteroidetes indicates similar activities are common in the ocean. The here presented data show that certain marine bacteria are genetically and biochemically variable enough to access parts of structurally diverse xylans from terrestrial plants as well as from marine algal sources. |
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