Griseofulvin: A potential agent of chromosomal segregation in cultured cells

The fungicidal compound griseofulvin (GF) induces abnormalities in nuclear division in mammalian cells cultured in vitro. For these properties it has been studied as a potential agent of chromosomal segregation. A marked effect on the dynamics of chromosomal complements was observed both on diploid and heteroploid cell lines, including hybrids produced by fusion. After treatment for three days with doses ranging from 40 to 60 μg/ml, according to the cell type, a tendency to a doubling of the chromosomal set was evident. When cells were allowed to recover in normal medium in the absence of GF a scattering of the distribution of the chromosomal numbers occured. After removal of the drug a selective advantage of the double chromosomal complements was observed on prolonged cultures. The possibility of using GF to induce chromosomal segregation for linkage studies and for chromosomal assignment is discussed.