Asparagine biosynthesis by Oryza sativa seedlings |
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Authors: | Tetsuo Kanamori Hideaki Matsumoto |
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Institution: | Department of Agricultural Chemistry, Kyoto University, Kyoto 606, Japan |
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Abstract: | l-Aspartate-U-14C] was quickly metabolized in rice seedlings into amino acids, organic acids and sugars. On feeding simultaneously with ammonium for 2 hr, about 1% of the total soluble radioactivity was recovered as asparagine. Major amino acids labelled were aspartate, glutamate, glutamine and alanine in both shoots and roots. On the other hand, on feeding l-aspartate-U-14C] to rice seedlings precultured in an ammonium medium, asparagine accounted for 35% of the total soluble radioactivity in the roots. Different labelling patterns in amino acids from those of non-precultured tissues were observed, and the main amino acids labelled in this case were asparagine and γ-aminobutyrate in the roots; glutamate, asparagine and glutamine in the shoots. It was observed in the roots that this increase of asparagine labelling was associated with a decrease of label in glutamate. |
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Keywords: | Gramincae rice ammonium nutrition asparagine biosynthesis |
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