癫痫发作敏感大鼠前深梨状皮层T区神经病理观察

目的和方法：采用颞叶癫痫红藻氨酸（kainic acid,KA)模型,制备癫痫发作敏感大鼠,并分别以硫瑾染色和GFAP（神经胶质原纤维酸性蛋白,glial fibrilary acidic protein)免疫组化方法检测前深梨状皮层T区（area tempestas,AT)内神经元损伤及星形胶质细胞增生情况,并与经蝎毒（scorpion venom,SV)处理后癫痫发作敏感性明显降低的大鼠进行比较。结果：与对照组比较,癫痫敏感动物前深梨状皮层T区锥体细胞数目明显减少,GFAP免疫反应阳性星形胶质细胞数目明显增加,染色强度明显增强,（P＜0．05）以剂量为100mg/kg/日的蝎毒给予动物连续灌胃三周,可明显降低其癫痫发作敏感性（P＜0．05）,而脑内梨状皮层T区锥体细胞脱失减轻,GPAP免疫反应活性未见明显增强。结论：推测梨状皮层T区硬化（神经元脱失,星形胶质细胞增生肥大）很可能是癫痫发作敏感性长期存在的重要原因。

Neuropathologic investigation of area tempestas sclerosis in epileptic sensitive rats

Aim and Methods:The relation between AT sclerosis(loss of neurons and proliferation of astrocytes)and long-lasting epileptic susceptibility was investigated by thionine staining,GFAP immunohistochemistry and observing the behavior of rats,after scorpion venom(SV) or normal saline(NS) administrated for three week. Results: Compared with NS+NS group,both the loss of neurons and proliferation of astrocytes were very marked in KA+NS group (epileptic susceptible rats) (P<0.05), but those changes were not visible in KA+NS group(epileptic nosusceptible rats).Conclusions: It suggested that AT sclerosis may be one of important reasons of the long-lasting epileptic susceptibility.