Differential precipitation and zinc chelate chromatography purification of biologically active HTLV-I Tax1 expressed in E. coli |
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| Authors: | P F Lindholm S J Marriott S D Gitlin J N Brady |
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| Affiliation: | Laboratory of Molecular Virology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892. |
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| Abstract: | ![]()
A protocol which involves sequential ammonium sulfate precipitation and zinc chelate chromatography to purify the HTLV-I Tax1 protein expressed in E. coli is described. The final Tax1 product is greater than 90% pure and the yield is approximately 1 mg per liter of liquid culture. The purified Tax1 protein is biologically active in indirect in vitro DNA binding assays and cellular NF-kB induction experiments. |
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