LincRNA AC027700.1在小鼠蜕膜化中的表达研究

长链非编码RNA(long non-coding RNA,lncRNA)是一类长度大于200 nt、不具有蛋白编码潜能的RNA分子.在细胞生长发育、物质代谢以及疾病等的发生发展过程中起关键调控作用,但在蜕膜化相关领域研究报道较少.为了探究lincRNA AC027700.1在早孕小鼠子宫内膜中的表达规律,初步探讨AC0...

The expression of lincRNA AC027700.1 in mouse decidualization

Long non-coding RNAs(lncRNAs),which belong to the non-protein-coding RNAs,are greater than 200 nt in length.Although they have been found to play crucial roles in the regulation of cell growth and development,cell metabolism and the development of diseases,they are rarely reported in decidualization.The objective of our study is to explore the expression of lincRNA AC027700.1 in the endometrium of early pregnant mice and its role in decidualization.The expression of AC027700.1 in uterine tissues at implantation sites and inter implantation sites on the 6th day of pregnancy were detected by qRT-PCR.The relative expression of AC027700.1 in an in vivo model of induced decidualization in pseudopregnant mice and in in vitro model of induced decidualization in primary stromal cells and nucleus/cytoplasmic fractions were detected by qRT-PCR.GO and KEGG analysis of downstream target genes were performed by GOseq and KOBAS,respectively.The results show that AC027700.1 expression is significantly increased in tissues at implantation sites on the 6th day of pregnancy and in decidualized endometrial tissues and stromal cells.Furthermore,AC027700.1 localizes in the nuclear fraction and the downstream targeted genes are mainly involved in autophagy,cell cycle and RNA transport pathways.This study revealed that lincRNA AC027700.1 may be involved in decidualization of endometrium in early pregnancy,but the specific role and regulatory mechanism remain to be further studied.