Regulation of polyamine synthesis in human hepatocytes by hepatotrophic factor augmenter of liver regeneration |
| |
Authors: | Dayoub Rania Thasler Wolfgang E Bosserhoff Anja K Singer Thomas Jauch Karl-Walter Schlitt Hans J Weiss Thomas S |
| |
Institution: | Department of Surgery, University of Regensburg Hospital, Germany; Center for Liver Cell Research, University of Regensburg Hospital, Germany. |
| |
Abstract: | Different stages of liver regeneration are regulated by a variety of factors such as the liver growth associated protein ALR, augmenter of liver regeneration. Furthermore, small molecules like polyamines were proven to be essential for hepatic growth and regeneration. Therefore, using primary human hepatocytes in vitro we investigated the effect of ALR on the biosynthesis of polyamines. We demonstrated by HPLC analysis that recombinant ALR enhanced intracellular hepatic putrescine, spermidine, and spermine levels within 9-12h. The activation of polyamine biosynthesis was dose dependent with putrescine showing the strongest increase. Additionally, ALR treatment induced mRNA expression of ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase, both key enzymes of polyamine biosynthesis. Further, ALR induced c-myc mRNA expression, a regulator of ODC expression, and therefore we assume that ALR exerts its liver regeneration augmenting effects through stimulation of its signalling pathway leading in part to enhanced polyamine synthesis. |
| |
Keywords: | Liver regeneration Polyamines Ornithine decarboxylase Hepatotrophic growth factor Augmenter of liver regeneration Immediate early gene c-myc |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|