Complete stoichiometry of free NADPH oxidation in liver microsomes |
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Authors: | A A Zhukov A I Archakov |
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Institution: | Department of Biochemistry, Medico-Biological Faculty, 2-nd Moscow Medical Institute, Ostrovityanova Ia, Moscow, USSR |
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Abstract: | The stoichiometry of free NADPH oxidation in phenobarbital induced rabbit liver microsomes was measured by means of registering the rates of NADPH, H+ and O2 consumption and and H2O2 production. ratio is approximately I indicating that about half H2O2 results from dismutation, the second half being formed directly. ΔNADPH:ΔH2O2 and ΔO2:ΔH2O2 ratios exceed I and therefore another product of the reaction is water. The fact that the ratio (ΔNADPH-ΔH2O2):(ΔO2-ΔH2O2) is 2 allows one to consider direct 4-electron O2 reduction as the major way of water formation rather than endogenous substrate hydroxylation. |
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