Hepatic distribution of iron, copper, zinc and cadmium-containing proteins in normal and iron overload mice |
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Authors: | Yan Zhang Bai Li Chunying Chen Zhonghong Gao |
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Institution: | (1) Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China;(2) Department of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, 430074, People’s Republic of China;(3) National Center for Nanoscience and Technology, Beijing, 100190, People’s Republic of China;(4) Division of Nitric Oxide and Inflammatory Medicine, E-Institutes of Shanghai Universities, Shanghai, 201203, People’s Republic of China; |
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Abstract: | Subcellular distribution of metal-containing proteins of Fe, Cu, Zn and Cd were determined in the liver samples of iron overload
mice by size exclusion high performance liquid chromatography with on-line coupling to UV and inductively coupled plasma mass
spectrometry. Collision cell techniques was used to remove polyatomic interferences for some elements, such as Fe. Comparative
molecular weight (MW) information of the elemental fraction was obtained within a retention time of 40 min. Fe was present
only in high-MW (HMW) protein; Cu, Zn and Cd were found in different MW proteins. It was also observed that these four elements
studied showed predominant association with HMW fractions. Moreover, compared with the normal group, we found that the contents
of these elements except Cu significantly increased and the distribution of some elements like Cd changed in iron overload
mouse liver. It means that excessive iron accumulation in vivo may affect the metabolism of other element such as Zn and Cd. |
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Keywords: | Iron overload Metalloproteins Hepatic distribution SEC-HPLC-ICP-MS |
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