|
|||||
|
|
| 应用细胞透性化技术快速提取氧化葡萄糖杆菌胞内右旋糖酐糊精酶 | |
| 引用本文： | 王舒,毛相朝,张鲁嘉,邢艳陇,王华磊,魏东芝.应用细胞透性化技术快速提取氧化葡萄糖杆菌胞内右旋糖酐糊精酶[J].生物加工过程,2010,8(3):35-39. |
| 作者姓名： | 王舒 毛相朝 张鲁嘉 邢艳陇 王华磊 魏东芝 |
| 作者单位： | 1. 中国科学院,青岛生物能源与过程研究所,中国科学院生物燃料重点实验室,青岛,266101 2. 中国科学院,青岛生物能源与过程研究所,中国科学院生物燃料重点实验室,青岛,266101;中国海洋大学,食品科学与工程学院,青岛,266003 3. 华东理工大学,生物反应器工程国家重点实验室,鲁华生物技术研究所,上海,200237 4. 中国科学院,青岛生物能源与过程研究所,中国科学院生物燃料重点实验室,青岛,266101;华东理工大学,生物反应器工程国家重点实验室,鲁华生物技术研究所,上海,200237 |
| 基金项目： | 国家自然科学基金资助项目，国家重点基础研究发展计划(973计划)资助项目 |
| 摘 要： | 将细胞透性化技术应用到右旋糖酐酶的提取检测过程中,根据单因素实验和正交试验设计确定了从氧化葡萄糖杆菌(Gluconobacter oxydans)细胞中提取胞内右旋糖酐糊精酶(DDase)的最佳方法:甘氨酸(Gly)质量分数15%,Triton X-100体积分数1%,菌悬液OD600为0.5~6.0,pH为6.81,冰浴处理时间1 h,透性化试剂的提取效率可达到酶活最大值的90%以上。与超声波细胞破碎法相比,该方法条件温和,酶的释放率较高并易于大量试样的平行实验操作。 |
| 关 键 词： | 细胞透性化 氧化葡萄糖杆菌 右旋糖酐糊精酶 甘氨酸 |
Method for determinating dextran dextrinase from Gluconobacter oxydans by cell permeabilization |
|
| WANG Shu,MAO Xiang-zhao,ZHANG Lu-jia,XING Yan-long,WANG Hua-lei,WEI Dong-zhi.Method for determinating dextran dextrinase from Gluconobacter oxydans by cell permeabilization[J].Chinese Journal of Bioprocess Engineering,2010,8(3):35-39. | |
| Authors: | WANG Shu MAO Xiang-zhao ZHANG Lu-jia XING Yan-long WANG Hua-lei WEI Dong-zhi |
| Institution: | 1.Key Laboratory of Biofuels,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao 266101,China;2.College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;3.New World Institute of Biotechnology,State Key Laboratory of Bioreactor Engineering,East China University of Scienceand Technology,Shanghai 200237,China) |
| Abstract: | The study was focused on the application of disrupting Gluconobacter oxydans to release dextran dextrinase（DDase） by cell permeabilization.Kind of agents,solution concentration,solution pH and the disruption time were studied,which affected the disruption efficiency.A rapid method for extracting and determinating of the DDase with glycine and Triton X-100 was presented.The optimal conditions for extraction were as follows： glycine concentration 15%,Triton X-100 concentration 1%,OD600 of Gluconobacter oxydans suspension 0.5 to 6,pH 6.81,extraction time 1 h.The extraction efficiency of permeability reagents could achieve over 90%.The method could obtain the higher breaking efficiency and be easier to scale up compared with the ultrasonic disruption. |
| Keywords: | cell permeabilization Gluconobacter oxydans dextran dextrinase glycine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |