MS1516是拟南芥四分体形成和小孢子发育的必需基因

以前报道了雄性育性下降突变体ms1516，而且图位克隆的方法已将突变基因MS1516定位到拟南芥基因组第3条染色体上28kh的区间内。本文通过进一步的生物信息学分析，发现该定位区间内有一个与减数分裂有关的基因AtATM，而且等位实验结果表明rns1516和nfm0是等位突变体。细胞学分析结果表明，ms1516突变体在花药发育过程中产生多个不均等的小孢子，而且大多数的小孢子不能发育成成熟的花粉。DAPI染色的结果显示小孢子母细胞减数分裂过程中，染色体不能正常分离，对成熟花粉的扫描电镜观察结果发现突变体多数花粉形态异常。以上结果说明MS1516基因在小孢子形成和发育过程中具有重要作用。

MS1516 is Essential for Tetrad Formation and Microspore Development in Arabidopsis thaliana

Anthers in flowers produce via meiosis haploid microspores, which mature to become pollen. However, pollen development at the molecular level is still not very clear. For the purpose of gaining understanding of the molecular control of pollen development, an Arabidopsis partial male-sterility mutant ms1516 was reported previously, and the mutant gene MS1516 was mapped to a region of 28kb on chromosome 3 by a map-based cloning approach. Here, we report that further bioinformatics analysis revealed that there...