Panagrellus redivivus: failure to find evidence for the occurrence and biosynthesis of sialic acids. |
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Authors: | G Reuter J Aumann U Wyss H B Jansson R Schauer |
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Institution: | Biochemisches Institut, Universit?t Kiel, Federal Republic of Germany. |
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Abstract: | The occurrence of sialic acids in the free-living nematode Panagrellus redivivus was studied by periodate oxidation/3H]sodium borohydride reduction of about 10(7) nematodes. In parallel, the capability of sialic acid biosynthesis was examined by metabolic labeling of the same number of nematodes with N-3H]acetylmannosamine. In both experiments, radioactivity was incorporated into the nematodes. Mild acid hydrolysis, however, did not release radioactively labeled sialic acids or derivatives as tested by radio thin-layer chromatography, suggesting that P. redivivus does not contain or synthesize sialic acids. |
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