中红侧沟茧蜂寄生对寄主粘虫血淋巴糖类、脂类和蛋白质含量的影响

为揭示寄生蜂寄生对其寄主的生理调控机制, 室内对中红侧沟茧蜂Microplitis mediator寄生与未被寄生寄主粘虫Mythimna separata幼虫血淋巴中糖类、脂类和蛋白含量变化进行了测定。结果显示: 在滞育与非滞育条件下, 被寄生的粘虫血淋巴中糖原浓度均比未被寄生的粘虫高。滞育条件下寄生后12 d差异显著（P<0.05）, 被寄生粘虫糖原含量为7.93 μg/mL, 未被寄生粘虫糖原含量为4.70 μg/mL; 非滞育条件下寄生后6 d差异显著（P<0.05）, 被寄生粘虫糖原含量为14.35 μg/mL, 未被寄生粘虫糖原含量为5.47 μg/mL。海藻糖含量测定结果显示, 在滞育条件下寄生蜂对被寄生粘虫无明显影响, 而非滞育条件下影响效果差异显著（P<0.05）, 寄生后4 d被寄生粘虫海藻糖含量为46.82 μg/mL, 未被寄生粘虫含量为26.72 μg/mL。在滞育与非滞育两种条件下, 寄生与未被寄生寄主脂类和蛋白含量没有显著性差异。结果说明: 寄生蜂的存在使寄主血淋巴中的糖原含量增高; 非滞育条件是影响被寄生粘虫海藻糖含量变化主要因素; 粘虫对中红侧沟茧蜂的寄生表现相当强的适应性和忍受力。

Effects of Microplitis mediator(Hymenoptera:Braconidae)parasitization on levels of sugars,lipids and proteins in the haemolymph of Mythimna separata(Lepidoptera:Noctuidae)

 To explore the mechanism of physiological and biochemical effects of wasp parasitization on its hosts, levels of glycogen, trehalose, lipids and proteins in the haemolymph of host Mythimna separata larvae parasitized and unparasitized by Microplitis mediator were examined. The results showed that the content of glycogen in parasitized M. separata larvae was higher than that in unparasitized host larvae. Under diapause condition for 12 days, the content of glycogen in parasitized larvae reached to 7.93 μg/mL which was significantly higher than that in unparasitized hosts (4.70 μg/mL) (P<0.05). Under non-diapause condition for 6 days, the content of glycogen in parasitized larvae reached to 14.35 μg/mL which was significantly higher than that in unparasitized hosts (5.47 μg/mL) (P<0.05). The contents of trehalose were not significantly different between parasitized and unparasitized host larvae under diapause condition. But under non-diapause condition for 4 days, the content of trehalose (46.82 μg/mL) in parasitized larvae was significantly higher than that in unparasitized larvae (26.72 μg/mL). The contents of lipids and proteins were not significantly different between parasitized and unparasitized larvae under diapause or non-diapause conditions. The results suggest that: (1) the content of glycogen was higher in the haemolymph of larvae parasitized by M. mediator; (2) the non-diapause conditions are the main factors which influence the content of trehalose in parasitized larvae; (3) the larvae of M. separata possess higher ability of adaptation and tolerance to the wasp parasitism.