Association of leukotriene B4 with the cytoskeleton of rabbit neutrophils. Effect of chemotactic factor and phorbol esters |
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| Authors: | P H Naccache T F Molski P Borgeat R I Sha'afi |
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| Affiliation: | 1. Department of Pathology, University of Connecticut Health Center, Farmington, Connecticut, USA;2. Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut, USA;1. Groupe de Recherches sur les Leucotrienes, Centre Hospitalier de l''Universite Laval, Sainte Foy, Quebec, Canada. |
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| Abstract: | Following its addition to a suspension of rabbit neutrophils, leukotriene B4 is rapidly (less than 1 min) recovered from the cytoskeletal fraction (Triton X-100 insoluble pellet) of these cells. The association of leukotriene B4 with the cytoskeleton can be competed with by leukotriene B4 itself and by 20-OH leukotriene B4 but not by 20-COOH leukotriene B4. In addition, the preincubation of the cells with fMet-Leu-Phe or with phorbol 12-myristate 13-acetate, but not with 4 alpha-phorbol 12,13-didecanoate, results in a greatly decreased association of leukotriene B4 with the cytoskeleton. These results suggest that a specific association between the leukotriene B4 receptors and the cytoskeleton may be involved in signal transduction in the leukotriene B4 stimulated neutrophils. |
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| Keywords: | fMet-Leu-Phe Formyl-methionyl-leucyl-phenylalanine PMA Phorbol 12,13-myristate acetate 4 alpha-PDD 4 alpha-Phorbol 12,13-Didecanoate 5(S), 12(R)-dihydroxy-6,8,11,14-(cis,trans,trans,cis)-eicosatetraenoic acid |
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