| 枯草芽孢杆菌葡萄糖脱氢酶基因的克隆及其序列分析 |
| |
| 引用本文： | 乔建军,杜连祥. 枯草芽孢杆菌葡萄糖脱氢酶基因的克隆及其序列分析[J]. 工业微生物, 2001, 31(3): 23-24,28 |
| |
| 作者姓名： | 乔建军 杜连祥 |
| |
| 作者单位： | 天津轻工业学院食品工程系,天津,300222;天津轻工业学院食品工程系,天津,300222 |
| |
| 摘 要： | 根据Lampel报道的葡萄糖脱氢酶基因序列设计合成两条引物,以野生型枯草芽孢杆菌染色体DNA为模板,PCR扩增得到含有葡萄糖脱氢酶基因的大约780bp的DNA片段,将其克隆到pUC-T载体中。序列分析表明,克隆得到的葡萄糖脱氢酶基因含有783bp,编码261个氨基酸的蛋白质。得到的基因序列与文献报道的进行比较,其核苷酸同源率为75．5％,编码氨基酸序列的同源率为83．9％。
|
| 关 键 词： | 葡萄糖脱氢酶基因 PCR扩增 DNA序列分析 |
Cloning and sequencing of glucose dehydrogenase from B . subtilis |
| |
| QIAO Jian jun,DU Lian xiang. Cloning and sequencing of glucose dehydrogenase from B . subtilis[J]. Industrial Microbiology, 2001, 31(3): 23-24,28 |
| |
| Authors: | QIAO Jian jun DU Lian xiang |
| |
| Abstract: | Chromosomal DNA as template, gene of glucose dehydrogenase from Bacillus subtilis was cloned in plasmid pUC T by PCR. Followed by sequening, the sequence of the fragment from Bacillus subtilis had a little difference from the sequence of glucose dehydrogenase in other paper. Gene of glucose dehydrogenase was composed of 783 base pairs coded for glucose dehydrogenase of 261 amino acids. |
| |
| Keywords: | Bacillus subtilis glucose dehydrogenase PCR DNA sequencing |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
