对一株铜绿假单胞菌22种耐药基因的研究

目的研究铜绿假单胞菌多重耐药情况和相关机制。方法采用聚合酶链反应（PCR）法对一株多重耐药铜绿假单胞菌进行β-内酰胺酶基因、氨基糖苷类修饰酶（AMEs）基因、喹诺酮类耐药基因、耐消毒剂基因（qacE△1-sul1）和整合酶基因检测,并对VIM基因进行了测序。结果PCR扩增结果显示该菌株aac（6′）-Ⅰb、blaCARB、gyrA、oprD2、ant（2″）-Ⅰ、qacE△1-sul1、blaIMP-I、blaTEM、blaVEB、aac（3）-Ⅱ、ant（3″）-Ⅰ、intⅠ1、blaVIM基因均为阳性,而aac（3）-Ⅰ、aac（6′）-Ⅱ、blaGES、blaGIM、blaOXA-10群、blaPER、blaSPM、blaSHV、blaDHA基因均为阴性,VIM基因扩增产物测序后经BLAST同源性分析表明为VIM-2型。结论铜绿假单胞菌存在多重耐药基因。管壁涂有季胺类、双胍类消毒剂和磺胺的II代导管的抑菌效果需重新评价。

Study on 1 strain of Pseudomonas aeruginosa with multi-drug resistance gene

Objective To study the mechanism of multi-drug resistance of Pseudomortas aeruginosa. Methods The genes of multi-drug resistance including beta-lactamase, aminoglycasides modifying enzymes （AMEs） , disinfect resistance and sulfanilamide-resistance were determined by polymerase chain reaction（PCR）. The sequence of VIM gene was detected. Results The results of PCR indicated that aac（6＇）- Ⅰ b,blaCARB,g）rA,oprD2,ant（2″）- Ⅰ ,qacE△1- sull, blaIMP-Ⅰ,blaTEM,blaVEB,aac（5）-Ⅱ ,ant（3″）-Ⅰ ,int Ⅰ 1 and blaVIM were positive,while aac（3）-Ⅰ ,aac（6＇）-Ⅱ, blaGES, blaGIM, blaOXA- 10 group, b/aPER, blaSPM, b/aSHV and blaDHA genes were negative. The sequence of VIM PCR products of beta-lactamase genes was analyzed by BLAST and the result showed that it was VIM-2. Conclusion There are multi-drug resistance genes in the strain of Pseudomonas aeruginosa. The anti-bacterial effect of the second generation tube, of which the surface is imbrued with diamidines biguanidines and sulfanilamide need to be reevaluated.