Influence of polylysine on adhesion of fibroblasts to glass substrates visualized by total internal reflection microscopy |
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Authors: | Jan Hornung and Günter Fuhr |
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Institution: | (1) Humboldt-University Berlin, Institute of Biology, Membrane Physiology, Invalidenstrasse 43, D-10115 Berlin, Germany |
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Abstract: | The cell adhesion topography of mouse fibroblasts growing on glass substrates has been investigated. In order to compare cell adhesion on covered and uncovered glass, substrates were partly exposed to a solution with 0.1 mg/ml polylysine (300 kDa) for 15 min before incubation with cell suspension. After cultivation for 1, 3, 6, and 24 h their adhesion was visualised by total internal reflection microscopy. In the presence of polylysine, cells incubated for 1 h were strongly attracted to the substrate, leading to a typical cell adhesion topography characterised by round concavities under the ventral cell membrane with an approximate diameter of 1 μm. The cavity-surrounding rims were tightly bound to the glass surface. During further cell cultivation, the topography changed into a well-organised adhesion pattern with focal contact areas on the periphery of the cells. In contrast to the polylysine-mediated adhesion, cells growing on untreated surfaces did not exhibit the cavity-like topography at any stage of cultivation, but a more point spread adhesion with a dense clustering of contact-forming areas. |
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Keywords: | Total internal reflection microscopy Cell-substratum adhesion Polylysine 3T3 |
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