Isolation of the Corynebacterium glutamicum glnA gene encoding glutamine synthetase I |
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| Authors: | Marc Jakoby,Martin Tesch,Hermann Sahm,Reinhard Krä mer,reas Burkovski |
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| Affiliation: | Laboratoire ORSTOM de Microbiologie Anaérobies, Universitéde Provence, CESB-ESIL case 925, 163 avenue de Luminy, 13288 Marseille Cedex 9, France;Sanofi Recherche, Groupe Elf-Aquitaine, 31676 Labège Cedex, France;School of Biomolecular and Biomedical Sciences, Faculty of Science and Technology, Griffith University, Brisbane, Qld. 4111, Australia;Unitédes Entérobactéries, INSERM U199, Institut Pasteur, 75724 Paris Cedex 15, France;Département de Biologie, Universitéde la Méditerranée, 13288 Marseille Cedex 9, France |
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| Abstract: | ![]()
The Corynebacterium glutamicum glutamine synthetase I (GSI) structural gene glnA was cloned by a PCR approach using oligonucleotide primers derived from conserved amino acid sequences of the GSI proteins from various bacteria. Disruption or deletion of this gene in C. glutamicum led to a glutamine auxotrophic phenotype and complete loss of glutamine synthetase activity, indicating the key role of this enzyme in nitrogen metabolism. Additionally, indications for a second glutamine synthetase, GSII, were found. |
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| Keywords: | Haloanaerobiaceae Haloanaerobium Taxonomy Oil field |
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