| 响应面法优选牡丹果荚中丹皮酚和芍药苷提取工艺的研究 |
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| 引用本文： | 周启超,姚欢欢,陈凤丽,杜新琦,赵春建,王化,杨磊,汪贵斌,祖元刚.响应面法优选牡丹果荚中丹皮酚和芍药苷提取工艺的研究[J].植物研究,2014,34(2):271-275. |
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| 作者姓名： | 周启超 姚欢欢 陈凤丽 杜新琦 赵春建 王化 杨磊 汪贵斌 祖元刚 |
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| 作者单位： | 1.东北林业大学森林植物生态学教育部重点实验室，哈尔滨 150040；;2.南京林业大学森林资源与环境学院,南京 210037；;3.黑龙江省科学院自然与生态研究所，哈尔滨 150040 |
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| 基金项目： | “十二五”国家科技支撑计划课题:杜仲和喜树珍贵材用和药用林定向培育关键技术研究与示范(2012BAD21B05) |
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| 摘 要： | 以牡丹果荚为原料,采用响应面分析法对影响微波辅助提取牡丹果荚中芍药苷和丹皮酚的主要因素(料液比、微波功率、微波时间)进行优化。结果表明:微波辅助提取牡丹果荚中的芍药苷及丹皮酚的最佳提取工艺条件为:液料比10∶1、微波功率253 W、微波时间10 min,牡丹果荚芍药苷和丹皮酚的得率分别为2.92、0.91 mg·g-1。与传统提取法相比,微波辅助提取方法不仅提取时间短,原料使用量少,而且提取率高,是一个高效的提取方法。
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| 关 键 词： | 牡丹 果荚 芍药苷 丹皮酚 提取 响应面 |
Optimization of Microwave-assisted Extraction of Paeoniflorin and Paeonol from Peony Pod Using Response Surface Methodology |
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| Institution: | 1.Key Laboratory of Forest Plant Ecology,Ministry of Education,Northeast Forestry University,Harbin 150040；2.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037；3.Institute of Nature and Ecology,Heilongjiang Academy of Sciences,Harbin 150040 |
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| Abstract: | In this study, the response surface methodology (RSM) was used to optimize the main factors of microwave-assisted extraction for paeoniflorin and paeonol from peony seed pods, including liquid-solid ratio, microwave power and time. The optimum extraction conditions were as follows: liquid-solid ratio was 10∶1, microwave power was 253 W, microwave time was 10 min. Under these condition, the yields of paeoniflorin and paeonol were 2.92 and 0.91 mg·g-1, respectively. Compared with the traditional extraction method, microwave assisted extraction method could reduce extraction time, save raw material usage and enhance extraction rate, which was an efficient extraction method. |
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| Keywords: | Peony pod Paeoniflorin Paeonol extaction response surface |
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