FlAsH-based live-cell fluorescent imaging of synthetic peptides expressed in Arabidopsis and tobacco |
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| Authors: | Estévez José M Somerville Chris |
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| Institution: | Carnegie Institution, Stanford, CA 94305, USA. |
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| Abstract: | Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetracysteine (TC) sequence were expressed transiently in tobacco, and transiently and stably in Arabidopsis under control of a strong constitutive promoter Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT2 reagents. |
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