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Optimization and validation of a robust human T-cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses
Authors:Yun Lin  Humilidad F. Gallardo  Geoffrey Y. Ku  Hao Li  Gregor Manukian  Teresa S. Rasalan  Yinyan Xu  Stephanie L. Terzulli  Lloyd J. Old  James P. Allison  Alan N. Houghton  Jedd D. Wolchok  Jianda Yuan
Affiliation:1. Department of Molecular Microbiology and Immunology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA;2. Department of Child Health, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA;3. Department of Neurology, University of Missouri School of Medicine, M616 Medical Sciences Building, Columbia, MO 65212, USA;1. Department of Clinical and Molecular Medicine, Sapienza University of Rome, Rome, Italy;2. Department of Biomedicine, Institute of Translational Pharmacology, CNR, Rome, Italy;3. Department of Clinical Sciences and Translational Medicine, Tor Vergata University, Rome, Italy;4. Department of Experimental Medicine, Sapienza University of Rome, Rome, Italy;5. Laboratory of Molecular Medicine, Department of Systems Medicine, Tor Vergata University, Rome, Italy
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