Sequence complexity of polyadenylated RNA in human breast carcinoma cells |
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| Authors: | S K Arya |
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| Affiliation: | 1. The School of Chemical and Material Science, Shanxi Normal University, No. 1, Gongyuan Street, Linfen, 041004, China;2. Key Laboratory of Magnetic Molecules & Magnetic Information Materials Ministry of Education, Shanxi Normal University, Linfen, 041004, China;1. Basque Centre for Materials, Applications and Nanostructures (BCMaterials), UPV/EHU Science Park, Leioa, 48940, Spain;2. Macromolecular Chemistry Group (LQM), Department of Physical Chemistry, Faculty of Science and Technology, University of Basque Country (UPV/EHU), Leioa, 48940, Spain;3. Materials + Technologies Group, Department of Chemical and Environmental Engineering, Engineering College of Gipuzkoa, University of the Basque Country (UPV/EHU), Plaza Europa 1, Donostia-San Sebastian, 20018, Spain;4. IKERBASQUE, Basque Foundation for Science, Bilbao, 48013, Spain |
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| Abstract: | - 1.1. The sequence complexity of total cellular and polysomal poly(A)-containing RNA in human breast carcinoma cells was estimated to be respectively about 200,000 and 38.000 kilobases based on kinetics of RNA: cDNA hybridization.
- 2.2. Prolactin treatment of these cells moderately altered the apparent distribution of polysomal RNA sequences among the frequency classes.
- 3.3. Total cellular and polysomal poly(A)-containing RNA corresponded to about 4.8 and 1.6% of the single-copy DNA in these cells, respectively.
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