| 新型快速显微多道分光光度系统及其应用 |
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| 引用本文： | 籍涛,陈光炜,陈文心,黄耀熊. 新型快速显微多道分光光度系统及其应用[J]. 生物物理学报, 2001, 17(3): 599-604 |
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| 作者姓名： | 籍涛 陈光炜 陈文心 黄耀熊 |
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| 作者单位： | 1. 中山医科大学物理教研室
2. 暨南大学生物医学工程研究所 |
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| 基金项目： | 广东省自然科学基金(批准号:980692)和国务院侨办重点攻关项目基金资助项目 |
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| 摘 要： | 介绍一种新型快速显微多道分光光度系统。利用倒置显微镜、衍射光栅、线阵CCD等构成的显微多道分光光度系统，可对微区样品在350nm-800nm（波长分辨率为0．2nm)光谱波长范围内进行快速光谱检测，最快检测时间为1ms。利用该系统进行的人血红细胞内血红蛋白测量分析表明，该具有高灵敏度、快速、无扰、在位、多光谱显微测量的特点，在生命科学研究中动态监测细胞生命过程的变化。
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| 关 键 词： | 显微分光光度 多道光学系统 光谱 单个活细胞 应用 |
| 文章编号： | 1000-6737(2001)03-0599-06 |
| 修稿时间： | 2001-02-12 |
A NOVEL FAST MULTI-CHANNEL MICRO-SPECTROPHOTOMETER |
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| JI Tao,CHEN Guang-wei,CHEN Wen-xin,HUANG Yao-xong. A NOVEL FAST MULTI-CHANNEL MICRO-SPECTROPHOTOMETER[J]. Acta Biophysica Sinica, 2001, 17(3): 599-604 |
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| Authors: | JI Tao CHEN Guang-wei CHEN Wen-xin HUANG Yao-xong |
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| Affiliation: | JI Tao1,CHEN Guang-wei2,CHEN Wen-xin2,HUANG Yao-xong2 |
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| Abstract: | A novel fast micro multi-channel spectrophotometer is developed based on an inverted microscope with monochromator, linear array CCD, and a personal computer. This system can obtain absorption spectrum from a micro region of 1μm in diameter in the range of wave length from 350nm to 800 nm with resolution of 0.2nm within 1ms. The spectrum of hemoglobin in red blood cell, and the time course of the absorption spectrum of single red blood cell during hemolysis process obtained by the system show that the new spectrophotometer is of the ability of performing non-invasive, in situ, multi-channel measurement with high sensitivity and high speed. It is suitable for monitoring the instant molecular change of a single intact cell in response to the variation of its physiological and biochemical conditions. |
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| Keywords: | Micro-spectrophotometer Multi-channel Spectrum Single intact cell |
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