| 大孢虫花菌丝体多糖提取工艺优化及其测定方法优选 |
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| 引用本文： | 刘桂君,文华安.大孢虫花菌丝体多糖提取工艺优化及其测定方法优选[J].菌物学报,2006,25(4):624-632. |
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| 作者姓名： | 刘桂君 文华安 |
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| 作者单位： | 1. 中国科学院微生物研究所真菌地衣系统学重点实验室,北京,100080;中国科学院研究生院,北京,100049
2. 中国科学院微生物研究所真菌地衣系统学重点实验室,北京,100080 |
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| 摘 要： | 通过采用3,5-二硝基水杨酸法、蒽酮-硫酸法和苯酚-硫酸法测定大孢虫花PaecilomycestenuipesPeck菌丝体多糖含量,比较精密度、样品回收率、稳定性三个指标,得出最佳测定方法为蒽酮-硫酸法。根据一次一因素实验和Box-Behnken中心组合实验,应用SAS软件分析得出大孢虫花菌丝体多糖提取的最佳条件为:100℃沸水浴,提取2h,重复4次,料液比(菌丝体质量:蒸馏水体积)为1︰20,此条件下大孢虫花菌丝体多糖为4.12g/100g。
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| 关 键 词： | 3 5-二硝基水杨酸 蒽酮-硫酸 苯酚-硫酸 Box-Behnken中心组合实验 SAS软件分析 |
| 文章编号： | 1672-6472（2006）04-0624-0632 |
| 收稿时间： | 2006-04-06 |
| 修稿时间： | 2006-05-22 |
Optimization of extraction and determination of polysaccharide in mycelium of Paecilomyces tenuipes |
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| Authors: | LIU Gui-Jun WEN Hua-An |
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| Institution: | 1Key Laboratory of Systematic Mycology and Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Belting 100080;2 Graduate school of Chznese Academy of Sczences, Belting 100049 |
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| Abstract: | The polysaccharide content of Paecilomyces tenuipes mycelia was detected by anthrone-sulfuric acid method. One-factor-at-a-time method, Box-Behnken central composite design and SAS software were adopted to investigate the effects of different extraction methods on polysaccharide content of Paecilomyces tenuipes mycelia. The optimal condition of polysaccharide extraction was: water bath treatment(100℃) for two hours and four times replication, and weight of mycelium/volume of distilled water =1:20. Under optimal condition, the polysaccharide content of Paecilomyces tenuipes mycelia was 4.12g/100g. |
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| Keywords: | 3 5-dinitrosalicylic acid anthrone-sulfuric acid phenol-sulfuric acid Box-Behnken central composite design SAS software analysis |
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