Improved degradation of 4-chlorobiphenyl, 2,3-dihydroxybiphenyl, and catecholic compounds by recombinant bacterial strains |
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| Authors: | Ji-Young Kim Youngsoo Kim Kyoung Lee Chi-Kyung Kim |
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| Affiliation: | (1) Department of Microbiology and Biotechnology, Chungbuk National University, 361-763 Cheongju, Korea;(2) Research Institute for Genetic Engineering, Chungbuk National University, 361-763 Cheongju, Korea;(3) College of Pharmacy, Chungbuk National University, 361-763 Cheongju, Korea;(4) Department of Microbiology, Changwon National University, 641-773 Changwon, Korea |
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| Abstract: | ThepcbC gene encoding (4-chloro-)2,3-dihydroxybiphenyl dioxygenase was cloned from the genomic DNA ofPseudomonas sp. P20 using pKT230 to construct pKK1. A recombinant strain,E. coli KK1, was selected by transforming the pKK1 intoE. coli XL1-Blue. Another recombinant strain,Pseudomonas sp. DJP-120, was obtained by transferring the pKK1 ofE. coli KK1 intoPseudomonas sp. DJ-12 by conjugation. Both recombinant strains showed a 23.7 to 26.5 fold increase in the degradation activity to 2,3-dihydroxybiphenyl compared with that of the natural isolate,Pseudomonas sp. DJ-12. The DJP-120 strain showed 24.5, 3.5, and 4.8 fold higher degradation activities to 4-chlorobiphenyl, catechol, and 3-methylcatechol than DJ-12 strain, respectively. The pKK1 plasmid of both strains and their ability to degrade 2,3-dihydroxybiphenyl were stable even after about 1,200 generations. |
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| Keywords: | biodegradation recombinant DNA 4CBP 2,3-DHBP catecholic compounds |
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