Influence of Al3+ on the titer of spiramycin and effective components in fermentor |
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| Authors: | Zhen Li Fengxian Hu Heping Lv Jun Zeng |
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| Institution: | 1. The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China;2. Research and Development Department, Topfond Pharmaceutical Co., Ltd, Zhumadian, Henan, China |
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| Abstract: | Spiramycin is a multicomponent antibiotic, and different components have different antibacterial activities. In Streptomyces spiramyceticus 16-10-2, spiramycin II and spiramycin III (SPMII and SPMIII) are the main components, while spiramycin I (SPMI) needs to be controlled below 12%. Based on this, the influences of Al3+ on total spiramycin titer and components were investigated in this work. Those experiments were mainly performed in 15?L fermentor and Al3+ made a great improvement in spiramycin titer. The optimal adding concentration and adding time of Al3+ were 0.32?g/L at 12?hr. Under this condition, spiramycin titer was increased by 19.51% compared with the control. Moreover, the percentage of SPMII and SPMIII was increased by 7.14%. At the same time, the time of mycelia autolysis was lengthened. In addition, the specific activities of acetyl-CoA synthetase, acetate kinase, acetylphosphotransferase, and acylating enzyme were much higher than those of control. The content of acetic acid and succinic acid was beyond 3 and 4.5 times than that of control, respectively. |
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| Keywords: | Al3+ Streptomyces spiramyceticus 16-10-2 Spiramycin |
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