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Primary structure of the majorO-glycosidically linked carbohydrate unit of human von Willebrand factor |
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| Authors: | Bruno Samor Jean-Claude Michalski Claudine Mazurier Maurice Goudemand Pieter De Waard Johannes F G Vliegenthart Gérard Strecker Jean Montreuil |
| Institution: | (1) Laboratoire d'Hémostase, Centre Régional de Transfusion Sanguine, F-59012 Lille Cedex, France;(2) Laboratoire de Chimie Biologique (Unité Mixte de Recherche du Centre National de la Recherche Scientifique, No. 111), Université des Sciences et Techniques de Lille Flandres-Artois, F-59655 Villeneuve d'Ascq Cedex, France;(3) Department of Bio-organic Chemistry, University of Utrecht, Utrecht, The Netherlands |
| Abstract: | A reduced tetrasaccharide chain was obtained from human von Willebrand factor (vWF) by mild alkaline borohydride treatment. The purification of thisO-glycosidically-linked oligosaccharide was achieved by serial affinity chromatography on immobilized concanavalin A andLens culinaris agglutinin and finally gel filtration. Its structure was determined by a combination of methylation studies and 500 MHz1H-NMR spectroscopy to be: NeuAc( 2-3)Gal( 1-3)NeuAc(2-6)]GalNAc-ol.Abbreviations ConA
concanavalin A
- LCA
Lens culinaris agglutinin
- vWF
von Willebrand factor
- NeuAc
N-acetylneuraminic acid
- Gal
d-galactose
- GalNAc-ol
N-acetyl-d-galactosaminitol
- HMW
high molecular weight
- LMW
low molecular weight |
| Keywords: | von Willebrand factor concanavalin A O-linked carbohydrate 1 H-NMR FAB-MS |
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