Cytokinin metabolism in Phaseolus vulgaris L. |
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Authors: | M V Palmer R Horgan P F Wareing |
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Institution: | (1) Department of Botany and Microbiology, University College of Wales, SY23 3DA Aberystwyth, Dyfed, U.K.;(2) Present address: Institut für Obst-, Gemüse- und Weinbau, Universität Hohenheim (05700), Postfach 700562, D-7000 Stuttgart 70, Federal Republic of Germany |
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Abstract: | The major cytokinins in stems of decapitated, disbudded bean plants have been identified by enzymic degradation, Sephadex LH20 and reversed phase high performance liquid chromatography, and by combined gas chromatography-mass spectrometry as 6-(4-hydroxy-3-methylbut-trans-2-enylamino)-9- -D-ribofuranosylpurine (zeatin riboside), 6-(4-hydroxy-3-methylbutylamino)-9- -D-ribofuranosylpurine (dihydrozeatin riboside), and the 5 -phosphates of these compounds (zeatin ribotide and dihydrozeatin ribotide). Minor cytokinins in this tissue were tentatively identified as dihydrozeatin-O- -D-glucoside and zeatin ribotide-O- -D-glucoside. 8-14C-]Dihydrozeatin appeared to be rapidly metabolized to dihydrozeatin ribotide when supplied to segments of stems from decapitated plants. These results are discussed in relation to the metabolism and distribution of cytokinins in the whole plant.Abbreviations TEAB
triethyl ammonium bicarbonate
- UV
ultra-violet
- GCMS
gas chromatography-mass spectrometry
- HPLC
high performance liquid chromatography
- TMS
trimethyl silyl |
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Keywords: | Cytokinin (endogenous) metabolism Cytokinin (ribotides) Phaseolus |
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