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Cryopreservation of invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure |
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| Authors: | H Takagi N Tien Thinh O M Islam T Senboku A Sakai |
| Institution: | (1) Japan International Research Center for Agricultural Sciences, Okinawa Subtropical Station, 1091-1 Kawarabaru, Maesato, Ishigaki city, 907 Okinawa, Japan;(2) Asabucho 1-5-23, Kitaku, 001 Sapporo, Japan;(3) Present address: Nuclear Research Institute, Dalat, Viet Nam;(4) Present address: Plant Genetic Resources Center, Bangladesh Agriculture Research Institute, Bangladesh;(5) Present address: Agriculture, Forestry and Fisheries Research Council Secretariat, MAFF, Tokyo, Japan |
| Abstract: | Invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2M glycerol plus 0.4M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.Abbreviations DMSO Dimethylsulfoxide - EG ethylene glycol - LN liquid nitrogen - MS Murashige & Skoog medium (1962) - TDZ thidiazuron |
| Keywords: | Shoot tips Cryopreservation Vitrification Taro [Colocasia esculenta (L ) Schott ] Tropical crops |
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