Standard karyotypes ofAegilops uniaristata,Ae. mutica,Ae. comosa subspeciescomosa andheldreichii (Poaceae) |
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Authors: | B Friebe E D Badaeva K Kammer B S Gill |
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Institution: | (1) Wheat Genetics Resource Center, Department of Plant Pathology, Throckmorton Hall, Kansas State University, 66506-5502 Manhattan, KS, USA;(2) Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russia;(3) Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany |
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Abstract: | C-banding patterns and polymorphisms were analyzed in several accessions of the diploidAegilops speciesAe. uniaristata, Ae. mutica, andAe. comosa subsp.comosa and subsp.heldreichii, and standard karyotypes of these species were established. Variation in C-band size and location was observed between different accessions, but did not prevent chromosome identification. One accession ofAe. uniaristata was homozygous for whole-arm translocations involving chromosomes 1N and 5N. The homoeologous relationships of these chromosomes were established by comparison of chromosome morphologies and C-banding patterns to other diploidAegilops species with known chromosome homoeology. In addition, in situ hybridization analysis with a 5S rDNA probe was used to identify homoeologous groups 1 and 5 chromosomes. The present analysis permitted the assignment of allAe. mutica, comosa subsp.comosa, andAe. comosa subsp.heldreichii chromosomes, and three of the sevenAe. uniaristata chromosomes according to their homoeologous groups. The data presented will be useful analyzing genome differentiation in polyploidAegilops species. |
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Keywords: | Poaceae Aegilops uniaristata Ae mutica Ae comosa subsp comosa Ae comosa subsp heldreichii C-banding karyotypes |
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