In situ quantification of microcarrier animal cell cultures using near-infrared spectroscopy |
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| Institution: | 1. Laboratoire des Sciences du Génie Chimique, UPR CNRS 6811, Nancy-Université, 2 avenue de la Forêt de Haye, F-54505 Vandoeuvre-lès-Nancy Cedex, France;2. ThermoFisher Scientific, 91963 Courtaboeuf Cedex, France;3. Sanofi pasteur, 1541 avenue Marcel Mérieux, F-69280 Marcy L’Etoile, France;3. From the Institut für medizinische Physik und Biophysik, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany,;4. Institut für Biologie, Experimentelle Biophysik;5. Institut für Biologie, Biophysikalische Chemie, Humboldt-Universität zu Berlin, Invalidenstrasse 42, 10115 Berlin, Germany;1. Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China;2. Department of Ophthalmology, EENT Hospital of Fudan University, 83 Fenyang Road, Xuhui District, Shanghai 200031, China;1. Ecosystem Sciences, CSIRO, Australia;2. Research School of Biology, Australian National University, Australia;3. State Key Laboratory of Silkworm Genome Biology, Southwest University, China;4. Materials Science and Engineering, CSIRO, Australia;1. College of Aerospace Engineering, Chongqing University, Chongqing 400044, China;2. The State Key Laboratory of Mechanical Transmissions, Chongqing University, Chongqing, China;1. State Key Laboratory of Engines, Tianjin University, Weijin Road 92, Nankai District, Tianjin 300072, PR China;2. Brunel University London, Uxbridge UB8 3PH, United Kingdom |
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| Abstract: | In-line monitoring tools are still required to understand and control animal cell processes, particularly in the case of vaccine production. Here, in situ near-infrared spectroscopy (NIRS) quantification of components in culture media was performed using microcarrier-based cultivations of adherent Vero cells. Because microcarriers were found to interfere with NIRS spectra acquisition, a suitable and innovative in situ calibration was developed for bioreactor cultures. A reliable and accurate NIRS technique for the quantification of glucose and lactate was established, with a calibration standard error of 0.30 and 0.21 g l?1, respectively. The robustness of this method was evaluated by performing NIRS calibration with operating conditions similar to those of industrial processes, including parameters such as microcarrier concentrations, cell seeding states and changes in analyte concentration due to feed and harvest strategies. Based on this calibration procedure, the predicted analyte concentrations in unknown samples was measured by NIRS analyses with an accuracy of 0.36 g l?1 for glucose and 0.29 g l?1 for lactate. |
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