Synthesis and application of two light-sensitive copolymers forming recyclable aqueous two-phase systems |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China;2. Department of Applied Physics, Donghua University, Shanghai 200051, China;1. Faculty of Polymer Engineering, Sahand University of Technology, P.O. Box 51335-1996, Tabriz, Iran;2. Institute of Polymeric Materials, Sahand University of Technology, P.O. Box 51335-1996, Tabriz, Iran;1. Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada;2. Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada;1. Departamento de Química, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, CEP 35400-000 Ouro Preto, MG, Brazil;2. Grupo de Pesquisa em Química de Colóides, Instituto de Ciências Exatas, Universidade Federal de Alfenas (UNIFAL), Rua Gabriel Monteiro da Silva, 700, 37130-000 Alfenas, MG, Brazil;3. Departamento de Química, FACET, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Alta da Jacuba, CEP 39100-000 Diamantina, MG, Brazil;4. Laboratório de Soluções Analíticas Verdes, Departamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil;1. Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia;2. Biorefinery and Bioprocess Engineering Laboratory, Department of Chemical Engineering and Material Science, Yuan Ze University, No. 135 Yuan-Tung Road, Chungli, Taoyuan 320, Taiwan;3. School of Biosciences, Faculty of Science, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia;4. Biotechnology Research Centre, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor, Malaysia;5. Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;6. Chemical Engineering, School of Engineering, Monash University, 46150 Bandar Sunway, Selangor Darul Ehsan, Malaysia;7. Molecular Pharming and Bioproduction Research Group, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia |
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| Abstract: | Recycling aqueous two-phase systems (ATPS) are hopeful application techniques in bioseparation and biocatalysis engineering areas. In this study, two novel light-sensitive and reversible water-soluble copolymers were synthesized and used in recycling ATPS. Polymer PNBAC was polymerized by using N-isopropylacrylamide (NIPA), n-butylmethacrylate (BMA), acrylic acid (AA) and chlorophyllin sodium copper salt (CHL) as monomers. Copolymer PNDBC was synthesized by NIPA, 2-(dimethylamino) ethylmethacrylate (DMAEMA), BMA and CHL as monomers. The synthesis yields of two copolymers are 66.9% and 77.2%, respectively. It can be calculated from 1H NMR graph that the molar ratios of monomers in PNBAC (NIPA:BMA:AA:CHL) and PNDBC (NIPA:DMAEMA:BMA:CHL) was 82:4:1:10 and 16:4:3:1, respectively. They could be precipitated by laser irradiation in 488 nm with the least light density of 1.70 × 105 W/m2. The precipitate energy is 1.79 kJ and 3.52 kJ/g, respectively. Five batches of average recoveries of two copolymers in the ATPS are 96.6% and 97.4%. BSA and l-phenylalanine were partitioned in the novel PNDBC–PNBAC ATPS, and their partition coefficients were 2.1 and 0.52. By applying this ATPS to partition of penicillin, the best partition coefficient K is 4.25. |
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