Theanine synthesized by immobilizing Pseudomonas nitroreducens LY in nanofibrous membranes |
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| Institution: | 1. School of Life Sciences and Technology, Tongji University, No. 1239 Siping Road, Shanghai 200092, People''s Republic of China;2. College of Life Sciences, Nanjing Agricultural University, No. 1 Weigang Road, Nanjing 210095, People''s Republic of China;3. Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Department of Chemistry, Fudan University, Shanghai 200433, People''s Republic of China;1. Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Motooka, Fukuoka 819-0395, Japan;2. Biomass Engineering Program, RIKEN, 1-7-22, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan;3. Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan;4. Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka 812-8581, Japan;5. Center for Future Chemistry, Kyushu University, Fukuoka 819-0388, Japan;3. Department of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon 97239;4. Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina 27710;5. Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts 02118;1. Department of Chemical Engineering, Faculty of Engineering, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;2. Department of Computer Engineering, Faculty of Engineering, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;3. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;1. School of Chemical Engineering, Pusan National University, Busan, 46241, Republic of Korea;2. Advanced Biofuel and Bioproducts Process Development Unit, Lawrence Berkeley National Laboratory, Emeryville, CA, 94608, USA;1. Department of Agronomy, Iowa State University, Ames, IA, USA;2. Pasture Systems and Watershed Management Research Unit, Agricultural Research Service, USDA, University Park, PA, USA |
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| Abstract: | Pseudomonas nitroreducens LY was immobilized in nanofibers by an electrospinning process. We studied the impact of polymer concentration on the electrospinning process, and we also studied the viability of the immobilized cells and their impact factors. Furthermore, theanine was synthesized by the immobilized P. nitroreducens LY. Fibers with an average diameter of 220 nm were obtained with 8% (w/v) polyvinyl alcohol (PVA) and a potential of 17 kV. The highest survival rate (30%) of the immobilized P. nitroreducens LY was achieved at this condition. The P. nitroreducens LY in the fibers was stored at −20 °C for 3 months with no detectable decrease in cell viability. Using the nanofibers to immobilize P. nitroreducens LY, the theanine yield was 10.74 g/l with the conversion rate of sodium glutamate at 20.55%. Together, these results demonstrate the potential for application of advanced nanomaterials in microbial cell immobilizing technology by electrospinning. |
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