Candida albicans biofilm formation on peptide functionalized polydimethylsiloxane |
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| Authors: | Kristof De Prijck Nele De Smet Monika Rymarczyk-Machal Gonzalez Van Driessche Bart Devreese Tom Coenye |
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| Institution: | 1. Laboratory of Pharmaceutical Microbiology, Ghent University , Harelbekestraat 72, B-9000 , Ghent , Belgium;2. Laboratory of Polymer Chemistry and Biomaterials, Ghent University , Krijgslaan 281 S4bis, B-9000 , Ghent , Belgium;3. Laboratory for Protein Biochemistry and Biomolecular Engineering, Ghent University , K.L. Ledeganckstraat 35, B-9000 , Ghent , Belgium |
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| Abstract: | In order to prevent biofilm formation by Candida albicans, several cationic peptides were covalently bound to polydimethylsiloxane (PDMS). The salivary peptide histatin 5 and two synthetic variants (Dhvar 4 and Dhvar 5) were used to prepare peptide functionalized PDMS using 4-azido-2,3,5,6-tetrafluoro-benzoic acid (AFB) as an interlinkage molecule. In addition, polylysine-, polyarginine-, and polyhistidine-PDMS surfaces were prepared. Dhvar 4 functionalized PDMS yielded the highest reduction of the number of C. albicans biofilm cells in the Modified Robbins Device. Amino acid analysis demonstrated that the amount of peptide immobilized on the modified disks was in the nanomole range. Poly-d-lysine PDMS, in particular the homopeptides with low molecular weight (2500 and 9600) showed the highest activity against C. albicans biofilms, with reductions of 93% and 91%, respectively. The results indicate that the reductions are peptide dependent. |
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| Keywords: | biofilm Candida albicans peptide grafting Modified Robbins Device |
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