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Development of a CrN/Cu nanocomposite coating on titanium-modified stainless steel for antibacterial activity against Pseudomonas aeruginosa
Authors:T Elangovan  Rani P George  P Kuppusami  D Mangalaraj  Santanu Bera  E Mohandas
Institution:1. Thin Film and Nanomaterials Laboratory, Department of Physics , Bharathiar University , Coimbatore , 641 046 , India;2. Physical Metallurgy Division, Indira Gandhi Centre for Atomic Research , Kalpakkam , 603 102 , India;3. Center for Nano-Wear , Yonsei University , Seoul , 120 749 , Korea;4. Corrosion Science and Technology Group, Indira Gandhi Centre for Atomic Research , Kalpakkam , 603 102 , India;5. Physical Metallurgy Division, Indira Gandhi Centre for Atomic Research , Kalpakkam , 603 102 , India;6. Department of Nanoscience and Nanotechnology , Bharathiar University , Coimbatore , 641 046 , India;7. Water and Steam Chemistry Division, BARCF , Kalpakkam , 603 102 , India
Abstract:A relatively simple method was developed to fabricate CrN/Cu nanocomposite coatings using pulsed DC magnetron sputtering for application in antibacterial activity. These nanocomposite coatings were applied on titanium (Ti)-modified stainless steel substrata (D-9 alloy) and the antibacterial activity of these coating with respect to the Gram-negative bacterium Pseudomonas aeruginosa was investigated qualitatively and quantitatively. Scanning electron microscopy, epifluorescence microscope analyses, and total viable counts confirmed that inclusion of copper in the CrN/Cu nanocomposite coatings provided antibacterial activity against P. aeruginosa. The quantitative examination of the bacterial activity of P. aeruginosa was estimated by the survival ratio as calculated from the number of viable cells which formed colonies on nutrient agar plates.
Keywords:antibacterial activity  coating(s)  nanocomposite  Pseudomonas aeruginosa  sputtering  surface analysis
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