The Use of New Methylene Blue in Pseudomonas aeruginosa Biofilm Destruction |
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| Authors: | Mark Wainwright David A Phoenix Philip B Nickson Glyn Morton |
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| Institution: | 1. Department of Colour Chemistry , The University , Leeds, LS2 9JT, UK;2. Centre for Forensic Science , University of Central Lancashire , Preston, PR1 2HE, UK;3. Department of Biological Sciences , University of Central Lancashire , Preston, PR1 2HE, UK |
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| Abstract: | A Pseudomonas aeruginosa biofilm was produced in a model system using the bacterial strain NCIMB 8295, grown on silicone tubing (bore size 0.75 cm). Destruction of the biofilm was attempted using either ampicillin or a combination of white light (light dose=7.2 J cmm 2) and the phenothiazinium photosensitiser new methylene blue, and damage, both to extra-cellular polymeric substance (EPS) and to the organism, was monitored. It was found that although little damage to the EPS occurred with ampicillin, NMB caused both cell death and breakdown of the EPS, suggesting the use of photodynamic antimicrobial chemotherapy (PACT) in the disinfection of pathogenic biofilms, e.g. at external catheter surfaces. |
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| Keywords: | Biofilm New Methylene Blue Phenothiazinium Photosensitiser Pseudomonas Aeruginosa |
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