Real-time investigation of the muco-adhesive properties of Lactococcus lactis using a quartz crystal microbalance with dissipation monitoring |
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| Authors: | Doan Thanh Lam Le Sandrine Zanna Isabelle Frateur Philippe Marcus Pascal Loubière |
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| Affiliation: | 1. Université de Toulouse;2. INSA, UPS, INP;3. LISBP , 135 Avenue de Rangueil, F-31077, Toulouse , France;4. INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés , F-31400 Toulouse , France;5. CNRS, UMR5504 , F-31400 Toulouse , France;6. CNRS, LAAS , 7 avenue du colonel Roche, F-31077 Toulouse cedex 4 , France;7. CNRS, ITAV-UMS3039 , F31106 Toulouse , France;8. Université de Toulouse;9. UPS, INSA, INP, ISAE;10. UT1, UTM, LAAS, ITAV;11. , F-31077 Toulouse cedex 4 , France;12. Laboratoire de Physico-Chimie des Surfaces, CNRS/ENSCP UMR7045, école Nationale Supérieure de Chimie de Paris, Chimie ParisTech , 11 rue P. et M. Curie, F-75005 Paris , France;13. CNRS, UMR5504 , F-31400 Toulouse , France |
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| Abstract: | ![]()
This work was devoted to probe, at the entire population level, interactions between mucins and Lactococcus lactis, using QCM-D. Real-time monitoring of adsorption on polystyrene of PGM (Pig Gastric Mucin) and subsequent adhesion of L. lactis was performed for IBB477 and MG1820 strains. Measuring simultaneously shifts in resonance frequency and dissipation on the polystyrene-coated crystal demonstrated a two-phase process for PGM adsorption. XPS analysis confirmed the presence of adsorbed mucin. The Voigt-based model was used to describe the QCM-D outputs. The predicted thickness of the PGM layer was consistent with the AFM experimental value. Adhesion of L. lactis to bare or PGM-coated polystyrene was then monitored, in combination with DAPI cell counting. Positive frequency shifts were caused by adhering bacteria. The presence of adsorbed PGM strongly reduced bacterial adhesion. However, adhesion of IBB477 to the PGM coating was greatly increased in comparison with that of MG1820. Muco-adhesion may be a highly variable and valuable phenotypic trait among L. lactis strains. |
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| Keywords: | Lactococcus lactis mucin QCM-D, XPS viscoelasticity glycoprotein adsorption bacterial adhesion positive frequency shift |
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