Magnetic fields suppress Pseudomonas aeruginosa biofilms and enhance ciprofloxacin activity |
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Authors: | HMHN Bandara D Nguyen S Mogarala M Osiñski |
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Institution: | 1. College of Pharmacy, The University of Texas at Austin, Austin, TX, USA;2. Department of Electrical &3. Computer Engineering, Center for High Technology Materials, The University of New Mexico, Albuquerque, NM, USA |
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Abstract: | Due to the refractory nature of pathogenic microbial biofilms, innovative biofilm eradication strategies are constantly being sought. Thus, this study addresses a novel approach to eradicate Pseudomonas aeruginosa biofilms. Magnetic nanoparticles (MNP), ciprofloxacin (Cipro), and magnetic fields were systematically evaluated in vitro for their relative anti-biofilm contributions. Twenty-four-hour biofilms exposed to aerosolized MNPs, Cipro, or a combination of both, were assessed in the presence or absence of magnetic fields (Static one-sided, Static switched, Oscillating, Static + oscillating) using changes in bacterial metabolism, biofilm biomass, and biofilm imaging. The biofilms exposed to magnetic fields alone exhibited significant metabolic and biomass reductions (p < 0.05). When biofilms were treated with a MNP/Cipro combination, the most significant metabolic and biomass reductions were observed when exposed to static switched magnetic fields (p < 0.05). The exposure of P. aeruginosa biofilms to a static switched magnetic field alone, or co-administration with MNP/Cipro/MNP + Cipro appears to be a promising approach to eradicate biofilms of this bacterium. |
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Keywords: | Pseudomonas aeruginosa biofilms magnetic fields ciprofloxacin magnetic nanoparticles drug delivery |
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