Isolation and metagenomic characterization of bacteria associated with calcium carbonate and struvite precipitation in a pure moving bed biofilm reactor-membrane bioreactor |
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| Authors: | A Gonzalez-Martinez JC Leyva-Díaz A Rodriguez-Sanchez B Muñoz-Palazon A Rivadeneyra JM Poyatos |
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| Institution: | 1. Department of Civil Engineering, University of Granada, Granada, Spainagon@ugr.es;3. Department of Civil Engineering, University of Granada, Granada, Spain;4. Institute of Water Research, Granada, Spain;5. Department of Electronic and Computer Technology, University of Granada, Granada, Spain |
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| Abstract: | A bench-scale pure moving bed bioreactor-membrane bioreactor (MBBR-MBR) used for the treatment of urban wastewater was analyzed for the identification of bacterial strains with the potential capacity for calcium carbonate and struvite biomineral formation. Isolation of mineral-forming strains on calcium carbonate and struvite media revealed six major colonies with a carbonate or struvite precipitation capacity in the biofouling on the membrane surface and showed that heterotrophic bacteria with the ability to precipitate calcium carbonate and struvite constituted ~7.5% of the total platable bacteria. These belonged to the genera Lysinibacillus, Trichococcus, Comamomas and Bacillus. Pyrosequencing analysis of the microbial communities in the suspended cells and membrane biofouling showed a high degree of similarity in all the samples collected with respect to bacterial assemblage. The study of operational taxonomic units (OTUs) identified through pyrosequencing suggested that ~21% of the total bacterial community identified in the biofouling could potentially form calcium carbonate or struvite crystals in the pure MBBR-MBR system used for the treatment of urban wastewater. |
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| Keywords: | biofouling calcium carbonate biomineralization metagenomics pyrosequencing struvite |
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