Enhanced mineralization of pentachlorophenol by κ-carrageenan-encapsulated Pseudomonas sp. UG30 |
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| Authors: | M B Cassidy K W Shaw H Lee J T Trevors |
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| Institution: | (1) Department of Environmental Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada. Fax: 519 837 0442 e-mail: hlee@uoguelph.ca or jtrevors@uoguelph.ca, CA |
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| Abstract: | A pentachlorophenol(PCP)-degrading Pseudomonas sp. strain UG30 was encapsulated in κ-carrageenan for use in PCP degradation. Free and encapsulated cells were compared for
their ability to dechlorinate and mineralize 100–800 μg/ml sodium pentachlorophenate in broth. Dechlorination was measured
with a chloride ion electrode, and mineralization was measured by 14CO2 evolution from radiolabelled U-14C]PCP. Free and encapsulated Pseudomonas sp. UG30 cells mineralized up to 200 μg/ml and 600 μg/ml PCP, respectively, after 21 days. Encapsulation of UG30 cells provided
a protective effect, allowing dechlorination and mineralization of high levels of PCP to occur.
Received: 3 May 1996 / Received revision: 4 September 1996 / Accepted: 13 September 1996 |
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